Enhancing your imaging workflows and ultrastructral data using Biological EDS

Energy Dispersive X-ray Spectrometry (EDS) enables us to collect compositional information in combination with electron images. Biological EDS can measure and map elements within cells and tissues at the nanoscale, generating multi-colour images of cellular and subcellular features. The data generated provides ultrastructural context, mapping of elements within the biological samples and relative quantification of those elements. As an electron microscopist working in life science, it is easy to appreciate the added value of compositional information when characterising cell and tissue ultrastructure. Key learnings: • How to prepare your biological samples for EDS • How biological EDS complements electron microscopy as an imaging technique • Recognise how EDS enables you to navigate your sample easily and efficiently with AZtecLive • More ways to think about bioimaging, such as monitoring chemical variables with ultrastructural context and distinguishing features with and without labelling