Practical considerations for western blotting. Tips, Tricks, Facts, and Myths.

This presentation will highlight western blotting tips, tricks, facts, and myths that can significantly change how you look at western blotting. Western blotting is one of the keystone tools in cell biology and protein research. However, achieving reliable and repeatable quantitative western blot results can be difficult. This is because western blotting methods often vary widely based on the lab, where preferred methods are influenced by the equipment available and long held beliefs related to traditional protocols. This can lead to failed or sub-optimal experiments without a clear path for re-optimizing conditions. This wastes time and resources, and in some cases even produces incorrect scientific conclusions. Learn how to avoid these pitfalls and improve your western blots by attending this talk. For Research Use Only. Not for use in diagnostic procedures.