Spectral Cytometry Success: From Design Input to Data Output

The capabilities of flow cytometry instruments in terms of available lasers, detectors and number of measurable parameters have expanded tremendously over the years. Concomitant with that, there is an exponential increase in the number of fluorochromes available for flow cytometry, extending the limits of possibilities for new discoveries. Additionally, spectral flow cytometry collects the full spectrum, which increases the flexibility and the capability of spectral flow by enabling autofluorescence profiling and extraction, detection of more fluorochromes per laser line and increased panel size. Join our three-part series to gain a deeper understanding of spectral flow cytometry, learn how to apply these fundamentals for next-gen spectral cytometry, and see how one scientist used a 40-color spectral panel for deep immunophenotyping.