Regulatory T Cells In The Tissues: Characteristics And Importance Of Non-Lymphoid Tissue-Resident T Regulatory Cells

The tissues are the site of many of the most important immunological reactions, yet the biology of immunology in the tissues has remained relatively opaque. Recent studies have identified Foxp3+ regulatory T cells (Tregs) in several non-lymphoid tissues. These tissue-resident populations have been ascribed unique characteristics based on phenotypic differences from lymphoid Tregs, RNA-Seq profiles, and TCR usage. Using high-dimensional flow cytometric profiling, kinetic analysis and functional TCR testing using a flow cytometry-based barcoding system, we instead observed that there is a single pool of pan-tissue Tregs with little or no homing preference for their tissue of origin. While tissue Tregs constitute a single pool of broadly self-reactive activated Tregs that patrols non-lymphoid tissues, the protective impact on particular organs can be amplified by changing the tissue Treg niche size. Using a gene delivery system, we demonstrate strong protection from neuroinflammation across multiple neurological injury and disease models when the tissue Treg niche size is expanded in the brain.

Key topics discussed in this webinar will include:

• Characteristics of non-lymphoid tissue-resident Treg cells 
• Techniques used to study tissue-resident Tregs
• The importance of tissue-resident Tregs in protective responses and physiological homeostasis

Who should attend:

• Researchers focused on Treg phenotype and function
• Researchers interested in immune responses in tissues
• Anyone interested in flow cytometry applications

For Research Use Only. Not intended for use in diagnostic procedures.