Harnessing Full Spectrum Flow Cytometry And In Vivo Labeling

LOCATION, LOCATION, LOCATION: Harnessing Full Spectrum Flow Cytometry And In Vivo Labeling Techniques To Spatially Profile Murine Pulmonary Immune Cells In Health And Disease

The lung is a complex organ with a deeply heterogeneous cellular landscape. Routinely exposed to inhaled particulates including microbes, allergens, and environmental pollutants, lung cells possess unique characteristics that protect the host from infection and damage. Comprehensive cellular phenotyping of the lung is essential to deepen our understanding of respiratory diseases and the role that various cell types play in modulating lung structure, function, and overall host protection. Spectral flow cytometry is a vital tool for studying tissue responses at the single-cell level, however, important spatial information is lost during sample preparation. In this webinar, Kerry Hilligan will present a multifaceted spectral cytometry platform that incorporates in vivo staining techniques to retain important spatial information about immune subsets in the datasets. The platform additionally includes individual non-immune-, myeloid- and lymphoid-targeted panels, with a conserved 16-parameter backbone, to facilitate comprehensive profiling of all major lung cell subsets, including their activation status, functional properties, and tissue localization. Details of the fully optimized and validated 60-parameter platform will be shared with attendees along with strategies for panel development, data analysis, and interpretation to enable detailed investigation of pulmonary cells in health and disease.

Key topics discussed in this webinar will include:

• Inclusion of in vivo labeling techniques in experimental design to retain key spatial information about murine pulmonary immune populations
• Strategies for successful panel development and optimization working with a complex and heterogeneous tissue (e.g. incorporation of intracellular overnight staining techniques to dramatically improve marker resolution)
• Development and optimization of non-immune-, myeloid- and lymphoid-specific spectral panels with a conserved lineage marker backbone 
• Data handling strategies to analyze and interpret high dimensional datasets

Who should attend:

• Researchers interested in the immune landscape of the lung and response to respiratory diseases
• Scientists using tissue samples for high dimensional spectral flow cytometry
• Anyone interested in flow cytometry applications 

For Research Use Only. Not intended for use in diagnostic procedures.