Optimizing Full Spectrum Cell Sorting For Generating Viable And Functional Cells For Downstream Assays

Cell sorting is a crucial technique that serves as a preliminary step for numerous downstream applications, particularly in cancer research and regenerative medicine. However, sorting at low PSI values presents challenges, as reduced flow velocities can lead to random and uneven droplet formation. This issue becomes particularly pronounced when using a 130 micron (µm) nozzle, which, though rarely employed, is necessary for sorting large or fragile cells. The transition to this nozzle requires additional setup time and can impact overall platform efficiency due to prolonged equipment use.

In this webinar, we discuss the optimization of cell sorting with a 130 µm nozzle using the Cytek Aurora™ CS system. Sorting dissociated tumor tissue cells, such as those derived from human prostate biopsies, is complex due to cellular fragility, autofluorescence, and the variability of surface marker expression. These factors significantly influence cytometric profiles and sorting efficiency, particularly when the sorted cells are intended for downstream applications like organoid generation.

By leveraging cellular autofluorescence and increasing the discrimination of up to six distinct subpopulations, we refined our sorting approach to enhance viability and purity. Furthermore, we highlight how optimizing sorting pressure contributed to improved cell survival, a critical factor for successfully generating functional organoids. These findings emphasize the advantages of the Cytek Aurora CS system for intricate applications, paving the way for novel experimental strategies in cancer research and regenerative medicine.

Key topics discussed in this webinar will include:

• The advantages of full spectrum technology for cell sorting and improved discrimination of up to six distinct subpopulations using cellular autofluorescence
• Optimization of cell sorting with a 130 µm nozzle using the Cytek Aurora CS system, and how this improved sorting efficiency for fragile cells from dissociated tumor tissues
• The role of sorting pressure optimization in enhancing cell viability and the importance of viable sorted cells for functional organoid formation

Who should attend:

• Flow cytometry specialists and core facility staff looking to optimize sorting parameters for complex samples, including fragile tumor-derived cells

• Biomedical and clinical researchers interested in organoid generation and methods to improve cell viability during sorting

• Anyone interested in flow cytometry cell sorting applications
  

For Research Use Only. Not intended for use in diagnostic procedures.