Learn how to isolate lipid nanoparticles and their associated proteins in a label-free manner through density-gradient ultracentrifugation with quality control checks.
Explore how specific corona components impact the LNP delivery of RNA cargo.
Applications of density gradients for lipid nanoparticle protein corona isolation
About the Event
Lipid nanoparticles (LNPs) are clinically successful nonviral vehicles for RNA-cargo delivery, yet their interactions with biological systems remain incompletely understood. Upon exposure to biofluids, such as pooled human plasma, proteins spontaneously coat LNPs, forming an associated protein corona that defines the biological identity of LNPs. In this webinar, Elizabeth Voke will present a quantitative, label-free proteomics workflow developed to characterize the LNP protein corona. Using continuous density-gradient ultracentrifugation coupled with LC–MS/MS, her work accounts for the presence of endogenous nanoparticles in human plasma, enabling mechanistic insights into how corona composition modulates LNP uptake and mRNA expression. The findings, recently published in Nature Communications, reveal that specific proteins such as vitronectin and C-reactive protein can alter intracellular trafficking and mRNA expression.
Webinar Objectives:
Reserve your spot now to gain exclusive insights into how protein corona composition shapes LNP performance and RNA delivery efficiency.
2025-GBL-EN-108954-v1