High-Resolution Insights at 200 kV: A Twist to Bacterial Gyrase Inhibition through Non-Planar Analogs of the Antibiotic Cystobactamid

Cryo-electron microscopy (cryo-EM) has transformed biological research and drug discovery by enabling visualization of biomolecules in their native states and revealing critical structural insights. While high-resolution cryo-EM has traditionally depended on 300 kV cryo-TEM platforms, recent advances in 200 kV systems are redefining what is possible, delivering high-performance structural analysis with greater accessibility.

In this webinar, Prof. Dr. Jesko Köhnke will present his research using the 200 kV Thermo Scientific™ Glacios™ Cryo-TEM to investigate cystobactamids, a promising class of antibacterial compounds that inhibit DNA gyrase and topoisomerase IV. His team demonstrates how the strategic introduction of three-dimensional moieties significantly improved compound solubility while preserving potent, resistance-breaking activity against 188 clinical isolates. Cryo-EM analysis of a lead compound bound to the DNA gyrase of the WHO priority pathogen Acinetobacter baumannii revealed a new structural mode of target engagement, providing a framework to guide further antibiotic optimization.

Following Prof. Dr. Köhnke’s presentation, Dr. Lingbo Yu will introduce the newly launched Glacios 3 Cryo-TEM. Featuring integrated hardware and software innovations, the system is designed to enhance performance, productivity, and usability while reducing infrastructure requirements, broadening access to high-impact cryo-EM research.