qPCR 101: Introduction to Real-Time PCR

Presenter: Marcia Slater

This webinar will review the basics of Real-Time PCR. Topics include:

• How Real-Time PCR is different from traditional end-point PCR 

• Chemistry choices of TaqMan and SYBR Green; 1-step and 2-step 

• How a passive reference works to improve data quality 

• Selecting an endogenous control 

• The mathematics of using ddCt to determine changes in gene expression 

• Other tips for running impactful qPCR experiments

Real-Time PCR Assay Design Best Practices

Presenter: Kevin Kelly

Real-time PCR is an extraordinarily powerful tool that is widely used for a multitude of applications. But the quality of your real-time PCR data is only as good as the quality of your assay. This webinar discusses important considerations to keep in mind when it comes to assay design and will showcase several tools available through Thermo Fisher Scientific to help you to design and choose assays that enable your success performing real-time PCR.

Real-Time PCR Basics Part 2: How to Produce the Best Quality Data 

Presenter: Kevin Kelly 

Gene expression quantification is one of the most commonly performed, yet complex and demanding real-time PCR applications. But how do you know your results are accurate? This webinar discusses fundamentals of real-time PCR technology, as well as the challenges and vulnerabilities of gene expression experiments, and how to address them.

Set-Up and Analysis of a Real-Time PCR Experiment Using the QuantStudio3 or QuantStudio 5 Software

Presenter: Lihong Zhang

This webinar will demonstrate two methods to set-up and analyze gene expression data in the QuantStudio 3/5 instrument software: the relative standard curve method and the Delta-Delta-Ct method. It will include designating endogenous controls and reference samples. The software contains several features that allow a researcher to determine the quality of the amplification data. You will learn how to evaluate the quality of your qPCR data and how to use troubleshooting tools in the software when needed.

Analyzing qPCR Data in Thermo Fisher Connect

Presenter: Maura Andrews

This presentation will focus on Real-Time PCR gene expression analysis using Thermo Fisher Connect – a free online platform for cloud-based data storage, scientific analysis applications and peer collaboration tools. It offers advanced tools for analyzing Real-Time PCR data. In this session, we will walk through uploading data, creating projects for collaboration with peers, and online review of data using the Relative Quantification app.

Getting More From Your Real-Time PCR Machine: It Can Do So Much More Than Gene Expression!

Presenter: Min Le

This seminar reviews DNA, RNA, and protein applications that can be performed on Real-time PCR instruments. DNA applications include: Single Nucleotide Polymorphism (SNP) genotyping; Copy Number Variation (CNV) analysis; rare mutation detection (castPCR and digital PCR); and High Resolution Melt (HRM) analysis. RNA applications include: microRNA profiling; gene expression and lnc RNA analysis. Protein applications include: protein quantification assays and Protein Thermal Shift (PTS) analysis.

Strategies to Confirm Accuracy of Gene Expression Studies

Presenter: Zana Kapustina

As gene  expression  data  is  generated at  an  increasing  pace,  it  can  be  tempting  to  assume  that  “all  data  is valid.”  The  recent  retractions in peerreviewed  journals remind us of  the  value  of  confirming  data validity  prior  to  downstream  analysis and  publication. Zana Kapustina, doctoral  researcher  at  Thermo Fisher, will share  current techniques  to  confirm  gene  expression  validity  for qPCR  and  NGS gene expression  studies. 

Multiplexing in Real-Time PCR Experiments

Presenter: John Pfeifer

Multiplexing  in real-- Time PCR  Experiments time  PCR (Polymerase  Chain  Reaction)  is  the  amplification  and  detection  of  multiple gene  targets  in  the  same  reaction.  Multiplexing  is  more complex  than  only  selecting  reporter  dyes  for each probe. These  complexities  increase  with  the number  of gene  targets  being  multiplexed.  This presentation  focuses  on  what  is needed to  design  a  realtime  PCR  multiplex  and  test  it  to  ensure  it  is working  properly.