Development And Optimization Of A 36-Color Panel For Deep Immunophenotyping

In this webinar, we will present the development and optimization of a 36-color full spectrum flow cytometry panel for in-depth immunophenotyping of human T cells, with specific applications in adoptive cell therapy research. The panel supports comprehensive profiling of both peripheral blood and ex vivo expanded T cells relevant to CAR-T, TCR-T, and immune checkpoint inhibitor studies. Key features include high-resolution identification of CD4⁺ and CD8⁺ memory subsets, regulatory and helper T cell populations, and a broad activation-state coverage. Functional assessment is enabled through markers of proliferation and cytotoxicity, exhaustion, and senescence, supporting a deep understanding of T cell functionality and therapeutic potential. The panel also incorporates TCR specificity and transduction efficiency assessment and a PD-1 receptor occupancy component, providing an integrated approach to advanced T cell profiling using full spectrum flow cytometry.

Key topics discussed in this webinar will include:

• Development and optimization of a 36-color full spectrum flow cytometry panel with strategies to minimize spillover spreading error and enhance marker resolution
• Considerations for profiling both peripheral blood and ex vivo expanded T cells to ensure data consistency across sample types
• Applications of high-dimensional immunophenotyping in translational and cell therapy research

Who should attend:

• Researchers and scientists using spectral flow cytometry
• Immunologists studying T cell biology in health, disease, or cell therapy applications
• Core facility staff and flow cytometry specialists supporting advanced panel design

For Research Use Only. Not for use in diagnostic or therapeutic procedures.