Workshop Summary:  Metals play critical roles in biology, conferring unique reactivity, enabling challenging chemistry and redox reactions, and functioning as structural scaffolds. It has been estimated that 30-50% of all proteins bind a metal or metal cofactor. X-ray based techniques enable experiments that measure and monitor metal active site geometry and protein structure, as well as permitting elemental analysis to probe exactly what metals are present in a sample. In the Metals in Structural Biology Workshop we will emphasize the use of spectroscopic, X-ray crystallography, XFEL, and CryoEM methods to study metalloprotein structure and function, highlighting the capabilities uniquely available at SSRL and LCLS.

LOCATION: SLAC Building 51, Kavli Auditorium

This will be primarily an in-person workshop, but some talks may be available by zoom:  https://stanford.zoom.us/j/99712467317?pwd=Y3FXQ1pJTStsZlBaZStYNUpiNlVPZz09

Organizers:  Sarah Bowman; Jennifer Bridwell-Rabb

Morning Program: 

9:00-9:10 am        Introduction:  Jennifer Bridwell-Rabb and Sarah Bowman

9:10-9:35 am        Liliana Quintanar, Cinvestav Mexico: Copper, lens crystallins and cataract disease: What can we learn from X-ray absorption spectroscopy?

9:40-10:05 am      Jen Dubois, Montana State University: Making a good non-heme iron protein better through experiment and theory

10:10-10:35 am   Darya Marchany-Rivera, SLAC/SSRL: Anaerobic crystallography and in-situ UV-Vis Spectroscopy of proteins

10:40-11:00 am    Break

11:00-11:25 am   Yi Lu, University of Texas at Austin:  Understanding metals in biology by x-ray spectroscopic and crystallographic studies of artificial metalloenzymes

11:30-11:55 am   Yilin Hu & Markus Ribbe, University of California Irvine:  Nitrogenase – From Assembly to Catalysis

12:00-1:00 pm      Lunch Break

Afternoon Program:

1:00-1:15 pm        Riti Sarangi, SLAC/SSRL

1:15-2:30 pm        Panel Discussion:    

                               Gloria Borgstahl, University of Nebraska Medical Center

                               Aina Cohen, SLAC/SSRL

                               Katie Rush, Auburn University

                               Riti Sarangi, SLAC/SSRL

                               Jason Sidabras, Medical College of Wisconsin

                               Limei Zhang, University of Nebraska Medical Center           

2:30-2:50 pm        Break

2:50-3:15 pm        Hiroki Makita, LBNL

3:20-3:45 pm        Katie Graham, PNNL:  A Germin-Like Protein Bound to Iron

3:50-4:15 pm        Guillermo Calero, U Pittsburgh

4:20-4:45 pm        Amy Rosenzweig, Northwestern University:  CryoEM structures of particulate methane monooxygenase in a lipid bilayer