Metals play critical roles in biology, conferring unique reactivity, enabling challenging chemistry and redox reactions, and functioning as structural scaffolds. It has been estimated that 30-50% of all proteins bind a metal or metal cofactor. Synchrotron-based techniques enable experiments that measure and monitor metal active site geometry and protein structure, as well as permitting elemental analysis to probe exactly what metals are present in a sample. In the Metals in Structural Biology workshop we will emphasize the use of spectroscopic, X-ray crystallography, XFEL, and cryo-EM methods to study metalloprotein structure and function.
Zoom Meeting Passcode: 721594
Organizers:
Sarah Bowman, Hauptman Woodward Institute
Jennifer Bridwell-Rabb, University of Michigan
Jeney Wierman, SLAC National Accelerator Laboratory/SSRL
Agenda:
8:00 - 8:10 AM Introduction
8:10 – 8:40 AM High throughput micro-PIXE: an essential tool for metalloprotein research Elspeth Garman- University of Oxford
8:40 – 9:10 AM Caught in the act: visualizing the holocomplex of E. coli ribonucleotide reductase Kenny Kang Wellesley College
9:10 – 9:40 AM Chemical imaging at SSRL: Biological and Environmental Applications Jocelyn Richardson- SLAC National Accelerator Laboratory/SSRL
9:40 – 10:10 AM Break
10:10 – 10:40 AM Synchrotron Hard X-Ray Spectroscopy and Imaging Techniques in the Study of Alzheimer's Disease Kelly Summers- University of Saskatchewan
10:40 – 11:10 AM Role of Metal Ions in Carbonic Anhydrase Catalysis Chae Un Kim- Ulsan National Institute of Science and Technology
11:10 – 11:40 AM Architectural swapping activation mechanism for the human Fe-S cluster assembly complex David Barondeau- Texas A&M University
11:40 - 11:50 AM Remembrance of Deborah Zamble
11:50 - 12:00 AM Follow up discussion
Panelists:
- Doug Rees, California Institute of Technology
- Allen Orville, Diamond Lightsource
- Liliana Quintanar, Cinvestav
- Kelly Chacon, Reed College
- John Bacik, Cornell University