Time-resolved studies of biological macromolecules have yielded unprecedented details about structural transitions during biological function. Furthermore, these experiments have driven the development of new methods for sample triggering, delivery and data collection, as well as the commissioning of specialized experimental end-stations. While proteins that bind natural chromophores have been a major interest in the field, there is now increasing interest to broaden the ability of time resolved experiments to other targets. New strategies (including mixing, electric fields, temperature and pressure jumps, and syntheses of caged substrates) will be discussed along with potential classes of macromolecules that can now be studied by time resolved methods. A discussion of how these new strategies can be employed at specialized experimental end-stations at Synchrotrons and Free Electron Lasers will give the users an overview of current data collection capabilities and point the way forward for future developments.

Organizers:

James Fraser, UCSF Fraser Lab

Diana Monteiro, Hauptman Woodward Institute

Agenda:

    8:20- 8:30 AM Introductions 

    8:30- 8:55 AM Microfluidic Tools Facilitating Time-Resolved Crystallography Alexandra Ros, Arizona State  University

  8:55- 9:20 AM Timely solutions: Serial sample delivery and automation at SSRL and LCLS  Jennifer Wierman, SLAC National Accelerator Laboratory/SSRL

 9:20- 9:35 AM BREAK

9:35-10:00 AM Serial time-resolved crystallography at the T-REXX endstation (PETRA-III) David Von Stetten, European Molecular Biology Laboratory

10:00- 10:25 AM Time-resolved serial synchrotron crystallography for the dynamic characterization of enzymes Pedram Mehrabi, Max Planck Institute for the Structure and Dynamics of Matter                                   

10:25- 10:45 AM BREAK   

10:45- 11:10 AM Turning up the heat on dynamic proteins with multi-temperature and temperature-jump crystallography    Michael Thompson, University of California Merced                                      

11:10- 11:35 AM Push and pull, and see what happens—advanced in electric-field-stimulated X-ray crystallography  Doeke Hekstra, Harvard University

11:35-12:35 PM Q&A with Discussion